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| Iceland's Novator said on Wednesday it wanted to postpone discussion of the future of Finnish telecoms operator Elisa (ELI1V.Iceland's Novator said on
Wednesday it wanted to postpone discussion of the future of
Finnish telecoms operator Elisa (ELI1V."Novator continues to seek minority representation on
Elisa's Board of Directors, but now suggests that the further
discussion of the future corporate structure and strategy of
Elisa be postponed until after the EGM," the Icelandic investor
said in a statement.Novator, Elisa's largest shareholder, with an 11.Elisa's growth strategy and
splitting Elisa into a holding firm responsible for
international growth and a subsidiary responsible for daily
operations.Elisa Chairman Pekka Ketonen said Novator may have begun to
doubt its chances of success and was now seeking a new
opportunity to get its plans through."They may have thought it is more honourable to postpone
their plan, to seek for a better moment rather than take a
defeat at the meeting," Ketonen told Reuters, adding that
postponing the proposal was annoying for Elisa's management and
staff as it has already taken up a lot of time and resources."Now, if the matter is just left in the air and they begin
to seek a new chance to take their position, I don't think it is
according to good governance," Ketonen said."What are you doing here?"What are you doing here?Tata, VW to make cars in Thailand?Novator, the largest shareholder in Finnish telecoms operator Elisa (ELI1V.HE: Quote, Profile, Research), said on Wednesday it would propose postponing discussion of the future of the company until after Elisa's..."Novator, the largest shareholder
in Finnish telecoms operator Elisa (ELI1V.HE: Quote, Profile, Research), said on Wednesday
it would propose postponing discussion of the future of the
company until after Elisa's extraordinary meeting on Jan."Novator continues to seek minority representation on
Elisa's Board of Directors, but now suggests that the further
discussion of the future corporate structure and strategy of
Elisa be postponed until after the EGM," the Icelandic investor
said in a statement.Elisa, has
proposed changes, aimed to create shareholder value, that are
opposed by many shareholders, including pension funds, the
Finnish state and Nordea funds.The Finnish shareholders association has received more than
6,000 authorisations from small shareholders to vote against
Novator's revamp plans so far.Best photos from the past 24 hours."What are you doing here?"NYSE and AMEX quotes delayed by at least 20 minutes.Nasdaq delayed by at least 15 minutes.For other uses, see Elisa (disambiguation).Linked ImmunoSorbent Assay, or ELISA, is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample.The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality control check in various industries.In simple terms, in ELISA an unknown amount of antigen is affixed to a surface, and then a specific antibody is washed over the surface so that it can bind to the antigen.This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal.Performing an ELISA involves at least one antibody with specificity for a particular antigen.Between each step the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound.After the final wash step the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Older ELISAs utilize chromogenic substrates, though newer assays employ fluorogenic substrates with much higher sensitivity.Enzyme ImmunoAssay (EIA) is a synonym for the ELISA.Applications
2 History
3 Types
3.It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.The ELISA test, or the enzyme immunoassay (EIA), was the first screening test commonly employed for HIV.It has a high sensitivity.HIV antigens have been attached.If antibodies to HIV are present in the serum, they may bind to these HIV antigens.The plate is then washed to remove all other components of the serum.This secondary antibody is chemically linked in advance to an enzyme.It is also used in testing cats for the FIV disease.In radioimmunoassay, the radioactivity provides the signal which indicates whether a specific antigen or antibody is present in the sample.Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the surface of the container, i.ELISA
The steps of the general, "indirect," ELISA for determining serum antibody concentrations are:
Apply a sample of known antigen of known concentration to a surface, often the well of a microtiter plate.The antigen is fixed to the surface to render it immobile.These samples of known antigen concentrations will constitute a standard curve used to calculate antigen concentrations of unknown samples.Note that the antigen itself may be an antibody.This antibody will only bind to immobilized antigen on the well surface, not to other serum proteins or the blocking proteins.The plate is washed to remove any unbound detection antibody.Secondary antibodies, which will bind to any remaining detection antibodies, are added to the wells.The sandwich ELISA provides a solution to this problem.ELISA may be run in a qualitative or quantitative format.In quantitative ELISA, the optical density or fluorescent units of the sample is interpolated into a standard curve, which is typically a serial dilution of the target.ELISA, is used to detect sample antigen.The steps are as follows:
Prepare a surface to which a known quantity of capture antibody is bound.Wash the plate, so that unbound antigen is removed.Measure the absorbance or fluorescence or electrochemical signal (e.The image to the right includes the use of a secondary antibody conjugated to an enzyme, though technically this is not necessary if the capture antibody is conjugated to an enzyme.The major advantage of a sandwich ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.Without the first layer of "capture" antibody, any proteins in the sample (including serum proteins) may competitively adsorb to the plate surface, lowering the quantity of antigen immobilized.Competitive ELISA
A third use of ELISA is through competitive binding.The steps for this ELISA are somewhat different than the first two examples:
Unlabeled antibody is incubated in the presence of its antigen.The plate is washed, so that unbound antibody is removed.The more antigen in the sample, the less antibody will be able to bind to the antigen in the well, hence "competition."The secondary antibody, specific to the primary antibody is added.For competitive ELISA, the higher the original antigen concentration, the weaker the eventual signal.The labeled antigen competes for primary antibody binding sites with your sample antigen (unlabeled).Last accessed April 16, 2007.Food and Drug Administration.Last accessed April 16, 2007.YALOW R, BERSON S (1960)."Immunoassay of endogenous plasma insulin in man".Engvall E, Perlman P (1971).Van Weemen BK, Schuurs AH (1971).This page was last modified 02:58, 3 January 2008.All text is available under the terms of the GNU Free Documentation License.The shareholders of Elisa Corporation are invited to an Extraordinary General Meeting to be held on Monday 21 January 2008 at 14 p.Elisa Decided on The Issue of Stock Options 18.Notice Pursuant to the Finnish Securities Market Act, Chapter 2, Section 10 Regarding Change in Ownership 13.Rationalisation measures for Elisa units 04.The ELISA is a fundamental tool of clinical immunology, and is used as an initial screen for HIV detection.An HIV ELISA, sometimes called an HIV enzyme immunoassay (EIA) is the first and most basic test to determine if an individual is positive for a selected pathogen, such as HIV.The test is performed in a 8 cm x 12 cm plastic plate which contains an 8 x 12 matrix of 96 wells, each of which are about 1 cm high and 0.The next page illustrates how an HIV ELISA is performed.As recommended by
The Washington Post!Get Elisa Zied's new book now!If you want to find out what good health means, you should read this book.Feed Your Family Right, by registered dietitian Elisa Zied...With about 40 recipes and lots of practical advice, this would be an excellent resource for any parent trying to understand his or her own eating issues and get the kids off to a better start.Elisa is a Contributing Editor for Redbook magazine.Real Life, Healthy Life.She also provides content for both the magazine and online program, and works weekly with the three makeover participants.Read the latest issue of Elisa's eNewsletter ...Elisa Zied has been honored with the New York State Dietetic Association's Media Excellence Award.Elisa Zied is a registered dietitian, a New York state certified nutritionist and a spokesperson for the American Dietetic Association.Copyright (c) 2007 Elisa Zied.So I Married A Vampire has been nominated for a Night Owl Romance N.Readers can vote once daily until October 31st.The Upcoming page has been updated with a blurb for Bottled Up, a recent sale to Changeling Press.Damage Control is now available from Samhain Publishing. |
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![Elisa : [1997] -ristampa 1998] Pipes and flowers](/covers/11/11509/alb_61737_th.jpg "Elisa : [1997] -ristampa 1998] Pipes and flowers")